You should never blow the liquid out of the tip of syringes or pipettes. It is very difficult to transfer the mass of the blown out drop to a new container. Therefore, it is very important to use the appropriate tip for the volume of liquid you intend to transfer. However, this tip is not a plunger and should not be used to pressurize or squeeze liquid. Why don't you blow the liquid out of the tip of a pipette?
There are a few tips available in the market that can prevent this problem. They should be used when working with samples with unknown concentration. They should always be clean and well-rinsed before use. Another useful tip is the rubber tip or propipetter. In this way, you can easily control the volume of the liquid that you want to transfer. If you are working with a solution, you can lift your finger to release the liquid into another container.
There are two main types of pipettes: graduated and non-graduated. To choose the right one, make sure the graduations are far enough away from the tip. Then, insert the pipette tip into the solution and hold it there for at least three seconds. Don't blow out the liquid, but rather allow the liquid to run out under gravity. To achieve this, the tip of the pipette must remain in contact with the wall of the container for at least three seconds. Do not blow the liquid out, and do not agitate it or shake it. If you have any doubts, fit the tip with a slight twisting motion.
A volumetric pipette is a tube used to deliver a precise volume of liquid. It resembles a large rolling pin and should not be blown out, so a small amount of liquid should remain in the tip. When transferring a volume with a volumetric pipette, make sure the tip is properly inserted into the tube.
When choosing the correct tip for a micropipette, the tip should not be submerged too deep in the liquid. This could cause the liquid to stick to the tip and cause more fluid to be delivered than you intended. The tip should be 1-2 mm below the surface of the liquid. This is the ideal level for the tip to release the liquid. You will want to avoid aspirating any air through the tip as this will inhibit aspiration.
When using a pipette, take care not to reattach the syringe without first cleaning the syringe. Pipettes can become damaged from improper use and should be replaced. Even the smallest mishandle could compromise the accuracy of the liquid dispensed. If you can't find an appropriate replacement, it might be time to replace the pipette.
To prevent corrosion and maintain the quality of your pipettes, store them properly. Make sure to remove the caps from syringes and pipettes before using them. Proper storage also prevents moisture from entering the tip. Lastly, don't let the pipettes sit in water because this will result in the liquid coming out of the tip. This will extend their life and prevent them from breaking down.
You don't need to be a rocket scientist to learn about why you shouldn't blow a pipette. Using a pipette is a simple task, and you probably already know the importance of doing it right. A small drop of liquid will stay in the pipette due to the surface tension of the liquid. This small drop will not affect the experimental value.
Whenever possible, change the tip of a pipette before starting a new sample. Pipettes should be changed regularly to prevent contamination. While pipettes may use the same tip for calibration, it is important to prime them with each solution as they move up or down in concentration. To avoid contamination, always change the tip before piping a sample of unknown concentration. You'll also have more control over the volume of fluid you pipette, so you won't waste your time and effort by blowing a pipette.
Don't blow a pipette when it has an open tip. Always remember that a pipette is not a plunger. The tip should be fully drained before you use it. You'll be amazed at the difference. Just be sure to use the correct tip size for the type of sample you're working with. It will ensure you don't waste precious time attempting to calibrate a pipette.
Make sure that you inspect each pipet before you start a new project. Some pipettes get stuck before Proposition 13, so you should check the age of your stowaway pipets before using them. You can do this by dating them with a method known as C-14 dating. However, most breaks are trivial. Look for minor chipping around the tip edge or even a fracture in the pipette's wall, which compromises the liquid path.
When using a volumetric pipette, you should hold the pipette with your hand about a quarter of an inch from the bottom of your beaker. Don't squeeze the bulb of the pipette while holding it in your right hand. If you are left-handed, squeeze the pipette's bulb with your thumb and index finger, allowing the solution to flow into the pipet. Make sure you do not blow it out.
When taking measurements, it is essential to pre-wet the tip of the pipette before aspirating. The angle of the pipette tip is very important because it can affect the accuracy of the measurement. The angle should be between 90 degrees and 45 degrees. Using the pipette in this way will ensure that the desired volume enters the tip and that the liquid dissipates fully. Aspirating at an angle will increase the volume by as much as 10%.
One common mistake that amateur users make is not submerging the tip of the pipette too far into the liquid. If the tip is too deep, liquid will stick to the tip and deliver more than desired. Always press the pipette tip at a depth that is just below the liquid's surface. Using the pipette at this depth will prevent aspiration. Soak up that knowledge and you'll be on your way to being a better scientist.